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KMID : 0613820090190040549
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Journal of Life Science
2009 Volume.19 No. 4 p.549 ~ p.552
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Analysis of Trans-splicing Transcripts in Embryonic Stem Cell
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Ha Hong-Seok
Kim Heui-Soo
Huh Jae-Won
Kim Dae-Soo
Park Sang-Je
Bae Jin-Han
Ahn Kung
Yun Se-Eun
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Abstract
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Genetic mutations by gene fusion result from chromosomal rearrangement, trans-splicing, and intergenic splicing. Trans-splicing is a phenomenon in which two pre-mRNAs grow together into one. We analyzed the trans-splicing products in embryonic stem cells. By using bioinformatic tools, 70 trans-splicing transcripts were identified. They are classified into 6 types according to fusion pattern: 5’UTR-5’UTR, 5’UTR-3’UTR, 3’UTR-3’UTR, 5’UTR-CDS, 3’UTR-CDS, CDS-CDS. The fusion products are more abundant in CDS regions than in UTR regions, which contain multiple intron numbers. Chromosome analysis showing gene fusion via trans-splicing indicated that chromosomes 17 and 19 were activated. These data are of great use for further studies in relation to fusion genes and human diseases.
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KEYWORD
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Fusion gene, trans-splicing, bioinformatics, genetic mutation, chromosome analysis
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